These immunoglobulin fractions were readily purified by DEAE-cellulose chromatography and Sephadex G-200 gel filtration. Naturally occurring hemagglutinins for sheep erythrocytes were found to be 2-ME-sensitive and present in the 19S immunoglobulin fraction. No evidence for any anamnestic response was found in these animals. Throughout the course of immunization 2-ME-sensitive antibody was associated with a 19S immunoglobulin fraction (4-5 mg/ml serum) while late in the course of immunization antibody was found also associated with a 7S immunoglobulin fraction (7-8 mg/ml serum). Lemon sharks immunized with bovine serum albumin produced two molecular forms of antibodies detectable by passive hemagglutination of antigen-coated, tanned sheep erythrocytes.
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